Integrated Publishing Toolkit(IPT)

free and open access to biodiversity data

NDFF Environmental DNA

最新版本 由 Dutch National Database of Flora and Fauna (NDFF) 發佈於 Mar 26, 2018 Dutch National Database of Flora and Fauna (NDFF)

Environmental DNA (eDNA) is a DNA-based research method used to establish the presence of a species in samples. The underlying principle is that all organisms living in water emit DNA via skin cells, faeces and urine. The presence of a target species can be established by collecting water samples and analysing them for DNA in a laboratory. Research has shown that DNA dissolves in water within 2 to 3 weeks. When a water sample indicates the presence of DNA of a species, this means habitation must have been recent.

Detection is carried out via a Polymerase Chain Reaction (PCR) in a laboratory , using species-specific primers. In all cases, a large number (8 to 12) of PCR reactions are carried out on each sample (replica samples). The number of reactions that indicate a positive result for the DNA of the target species indicates how much DNA the sample contains. The amount of DNA is causally related to the occurrence of the target species, but there are many factors that affect the disintegration and production of eDNA.

Dataset available via https://www.ndff.nl/english / serviceteamndff@natuurloket.nl

Positive observations without zeros.

1,340 records (March 2018)

NDFF protocol https://www.ndff.nl/overdendff/validatie/protocollen/10-002-environmental-dna-onderzoek/

下載

下載最新版本資源元數據的EML或RTF文字檔。

元數據EML檔 下載 在 English 中 (7 KB)
元數據RTF文字檔 下載 在 English 中 (6 KB)

版本

The table below shows only published versions of the resource that are publicly accessible.

Rights

研究者應尊重以下權利聲明。:

The publisher and rights holder of this work is Dutch National Database of Flora and Fauna (NDFF). This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: cd8b0309-7663-46fa-8f07-cd3db0335131。  Dutch National Database of Flora and Fauna (NDFF) 發佈此資源,並經由Netherlands Biodiversity Information Facility同意向GBIF註冊成為資料發佈者。

關鍵字

Metadata; Observation

聯絡資訊

資源建立者:

Ronald Laan

可回覆此資源相關問題者:

Dries Oomen

元數據填寫者:

Dries Oomen

與此資源的相關者:

散布者
Henk de Vries
Node manager NLBIF
NLBIF Netherlands Biodiversity Information Facility P.O.Box 9517 2300 RA Leiden NL

地理涵蓋範圍

Netherlands

界定座標範圍 緯度南界 經度西界 [50.736, 3.318], 緯度北界 經度東界 [53.521, 7.207]

分類群涵蓋範圍

all organisms living in water detectable by DNA via skin cells, faeces and urine.

Class  Amphibia (Amphibian)
Species  Misgurnus fossilis (European weatherfish)

時間涵蓋範圍

起始日期 / 結束日期 2007-07-19 / 2016-10-10

額外的元數據

替代的識別碼 https://ipt.ndff.nl/resource?r=10_002_environmental_dna