NDFF Environmental DNA

Latest version published by Dutch National Database of Flora and Fauna (NDFF) on Mar 26, 2018 Dutch National Database of Flora and Fauna (NDFF)

Environmental DNA (eDNA) is a DNA-based research method used to establish the presence of a species in samples. The underlying principle is that all organisms living in water emit DNA via skin cells, faeces and urine. The presence of a target species can be established by collecting water samples and analysing them for DNA in a laboratory. Research has shown that DNA dissolves in water within 2 to 3 weeks. When a water sample indicates the presence of DNA of a species, this means habitation must have been recent.

Detection is carried out via a Polymerase Chain Reaction (PCR) in a laboratory , using species-specific primers. In all cases, a large number (8 to 12) of PCR reactions are carried out on each sample (replica samples). The number of reactions that indicate a positive result for the DNA of the target species indicates how much DNA the sample contains. The amount of DNA is causally related to the occurrence of the target species, but there are many factors that affect the disintegration and production of eDNA.

Dataset available via https://www.ndff.nl/english / serviceteamndff@natuurloket.nl

Positive observations without zeros.

1,340 records (March 2018)

NDFF protocol https://www.ndff.nl/overdendff/validatie/protocollen/10-002-environmental-dna-onderzoek/


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The table below shows only published versions of the resource that are publicly accessible.


Researchers should respect the following rights statement:

The publisher and rights holder of this work is Dutch National Database of Flora and Fauna (NDFF). This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: cd8b0309-7663-46fa-8f07-cd3db0335131.  Dutch National Database of Flora and Fauna (NDFF) publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Netherlands Biodiversity Information Facility.


Metadata; Observation


Who created the resource:

Ronald Laan

Who can answer questions about the resource:

Dries Oomen

Who filled in the metadata:

Dries Oomen

Who else was associated with the resource:

Henk de Vries
Node manager NLBIF
NLBIF Netherlands Biodiversity Information Facility P.O.Box 9517 2300 RA Leiden NL

Geographic Coverage


Bounding Coordinates South West [50.736, 3.318], North East [53.521, 7.207]

Taxonomic Coverage

all organisms living in water detectable by DNA via skin cells, faeces and urine.

Class  Amphibia (Amphibian)
Species  Misgurnus fossilis (European weatherfish)

Temporal Coverage

Start Date / End Date 2007-07-19 / 2016-10-10

Additional Metadata

Alternative Identifiers https://ipt.ndff.nl/resource?r=10_002_environmental_dna